Vector Labs Ab04109-23.159 Anti-TNFR1 [Nb70], Rabbit IgG-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Nb70
Synonyms: TNFRI; Tumor necrosis factor RI; Tumor necrosis factor R1; Tumor necrosis factor receptor type 1-associated death domain protein; TNFR1-associated death domain protein; TNFRSF1A-associated via death domain; Nb 14; Nanobody 14
Amount: 100 μg
Application Codes Clone: ELISA; SPR; functional assays; in vivo
Shipping Temperature: Wet Ice
Origin Pub PMID: 25538244
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human TNFR1.
Application Notes: The original format of this antibody (VHH) was used in ELISAs to determine its binding affinity to soluble human TNFR1 (hTNFR1) and other proteins such as albumin, human TNFR2 (hTNFR2), and mouse TNFR1 (mTNFR1); a K_D of 7.709 nM was measured for hTNFR1. This antibody was tested in a HEK-2 Blue assay to evaluate its inhibitory effect on the signaling of human TNF (hTNF) through TNFR1; it was found to effectively inhibit hTNFR1 by competing with TNF for binding with an IC₅₀ value of 4.144 μM and with lymphotoxin alpha (LTa) with an IC₅₀ of 220 nM. This antibody's affinity for hTNFR1 was evaluated by SPR analysis; a K_D of 38.71 nM was measured. This antibody was used to create a trivalent nanobody by linking it to clones Nb 96 and Nb Albumin using flexible [G₄-S]₃ linkers, and the resulting nanobody (Nb Alb-70-96) — TNF Receptor-One Silencer (TROS) — was tested for various in vitro, in vivo, and ex vivo (Steeland et al., 2015; PMID: 25538244). The original antibody format was used to create another trivalent nanobody by linking it to clones Nb 14 and Nb Albumin using the same linkers, and the resulting nanobody (Nb 70-Alb-14) was used in vivo to study the effects of inhibiting matrix MMP8 and TNFR1 in sepsis using endotoxemia and CLP (cecal ligation and puncture; a surgical procedure commonly used in animal models to induce polymicrobial sepsis) experimental models (Steeland et al., 2018; PMID: 29095202).

Product Class:	Purified
Clone ID:	Nb70
Synonyms:	TNFRI; Tumor necrosis factor RI; Tumor necrosis factor R1; Tumor necrosis factor receptor type 1-associated death domain protein; TNFR1-associated death domain protein; TNFRSF1A-associated via death domain; Nb 14; Nanobody 14
Amount:	100 μg
Application Codes Clone:	ELISA; SPR; functional assays; in vivo
Shipping Temperature:	Wet Ice
Origin Pub PMID:	25538244
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human TNFR1.
Application Notes:	The original format of this antibody (VHH) was used in ELISAs to determine its binding affinity to soluble human TNFR1 (hTNFR1) and other proteins such as albumin, human TNFR2 (hTNFR2), and mouse TNFR1 (mTNFR1); a K_D of 7.709 nM was measured for hTNFR1. This antibody was tested in a HEK-2 Blue assay to evaluate its inhibitory effect on the signaling of human TNF (hTNF) through TNFR1; it was found to effectively inhibit hTNFR1 by competing with TNF for binding with an IC₅₀ value of 4.144 μM and with lymphotoxin alpha (LTa) with an IC₅₀ of 220 nM. This antibody's affinity for hTNFR1 was evaluated by SPR analysis; a K_D of 38.71 nM was measured. This antibody was used to create a trivalent nanobody by linking it to clones Nb 96 and Nb Albumin using flexible [G₄-S]₃ linkers, and the resulting nanobody (Nb Alb-70-96) — TNF Receptor-One Silencer (TROS) — was tested for various in vitro, in vivo, and ex vivo (Steeland et al., 2015; PMID: 25538244). The original antibody format was used to create another trivalent nanobody by linking it to clones Nb 14 and Nb Albumin using the same linkers, and the resulting nanobody (Nb 70-Alb-14) was used in vivo to study the effects of inhibiting matrix MMP8 and TNFR1 in sepsis using endotoxemia and CLP (cecal ligation and puncture; a surgical procedure commonly used in animal models to induce polymicrobial sepsis) experimental models (Steeland et al., 2018; PMID: 29095202).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews