Mfg Part Number
Ab04118-2.0
Vector Labs Ab04118-2.0 Anti-1,3-diketone hapten [33F12], Mouse IgG2a, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing GIX+ mice with a keyhole limpet hemocyanin (KLH)-coupled diketone hapten.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 33F12 |
| Synonyms: | Hapten 1,3-diketone; β-keto acids; beta keto acids |
| Amount: | 100 μg |
| Application Codes Clone: | Catalytic assays; Crystallization |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 9405338 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody was raised against 1,3-diketone hapten, but it is capable of catalyzing the decarboxylation of structurally related β-keto acids. |
| Application Notes: | This antibody efficiently catalyzes aldol and retro-aldol reactions and covalently binds 1,3-diketone hapten. The (kcat/KM)/kuncat is approximately 109 at pH 7.5 as measured by the consumption of substrates and production of aldol products (monitored using reversed-phase high-performance liquid chromatography (HPLC) and enzymatic assays) (Wagner et al., 1995; PMID: 8525368). This antibody efficiently catalyzed the decarboxylation of two hapten-related β-keto acids (marked 4 and 5 in scheme 1 in the publication) with significant rate enhancements (kcat/kuncat) of 4959 and 11,261, respectively (Björnestedt et al., 1996). The crystallization of the Fab version of this antibody revealed a binding pocket characterized by a narrow, elongated cleft, where the catalytic lysine residue is prominently located (Barbas III et al., 1997; PMID: 9405338). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |