Mfg Part Number
Ab04134-10.0

Vector Labs Ab04134-10.0 Anti-ALCAM [035-234], Human IgG1, lambda (100 μg)

Quick Overview
The original antibody was isolated by panning a phage display library against ALCAM.
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Product Class:Purified
Clone ID:035-234
Synonyms:CD166; CD166 antigen; Activated leukocyte cell adhesion molecule
Amount:100 μg
Application Codes Clone:FACS; ADCC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:scFv
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The antibody is specific for ALCAM. ALCAM (Activated leukocyte cell adhesion molecule)” is transmembrane protein, which has a molecular weight of 100 to 105 kDa and is composed of five extracellular Ig-like domains and the intracellular terminus having 32 amino acid, and short transmembrane region. It is an immunoglobulin superfamily molecule including ten N-bonding type sugar chain added sites. ALCAM is one of the adhesive molecules, is present on the activated leukocyte and is identified as a ligand molecule to CD6 molecule (which functions as a signal receptor in T cells). ALCAM also functions as an adhesion factor in homophylic (ALCAM-ALCAM) or heterophylic (ALCAM-CD6) interaction.
Application Notes:The antibody exhibited ADCC activity on cancer cells expressing ALCAM, such as CW2 and NCI-H441 cells. The IgG1 format of the antibody could detect ALCAM in different cancer lines (040520IT, CHOK1SV,HepG2, HLF, Caki-1, CCFRC1, ACHN, 293T, PC14, SKOv3, BT474) by FACS (US9388249B2).
More Information
Brand Vector Labs
UNSPSC 12161500