Mfg Part Number
Ab04153-10.3
Vector Labs Ab04153-10.3 Anti-Chikungunya virus [CAP5A-F6], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated from a phage Fab library created from the B-cells of CHIKV infected patients. The library was panned against retroviral (Gag core) and CHIKV (alphavirus capsid core) virus-like particles (VLPs) displaying the native form of CHIKV E2/E1.
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| Product Class: | Purified |
|---|---|
| Clone ID: | CAP5A-F6 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CHIKV; IM-CKV065 |
| Amount: | 100 μg |
| Application Codes Clone: | FC; SPR; neutralizing; PRNT |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for chikungunya virus. The epitope was mapped to an area of E2 encompassing domains A and B, and arches 1 and 2. Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus, an RNA virus in the alphavirus genus of the family Togaviridae. |
| Application Notes: | The binding affinity of the original format of the antibody to CHIKV VLPs was measured by surface plasmon resonance (Kd= 9.3 nM). The original antibody demonstrated high reactivity with E2/E1 presented on cells, as shown by flow cytometry. The original format of the antibody was tested in a cellular neutralization assay against CHIKVL, where showed an average IC50 of 170 ng/ml. The original antibody was tested for antiviral activity using native replication-competent CHIKV viruses. In a plaque reduction neutralization test assay, the antibody could neutralize CHIKV strain 37997 with a PRNT50 value of 81 ng/ml (US9902765B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |