Mfg Part Number
Ab04158-23.0
Vector Labs Ab04158-23.0 Anti-3-SLeX [E2-B4], Rabbit IgG, lambda (100 μg)
Quick Overview
Vector Labs Ab04158-23.0 Anti-3-SLeX [E2-B4], Rabbit IgG, lambda (100 μg)
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | E2-B4 |
| Synonyms: | B4; 3’Sulfo-Lewis X |
| Amount: | 100 μg |
| Application Codes Clone: | IHC; IB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises the 3’sulfo-βGal linkage found on the tetrasaccharide carbohydrate Sialyl LewisX (sLeX), usually found attached to O-glycans on the surface of cells. 3’-Sulfo-Lewis X (3-SLeX) is a glycan epitope that has been reported to act as a ligand for the adhesion molecules E- and L-selectin. The synthesis of 3-SLeX is mediated by the enzyme galactose-3-O-sulfotransferase (GAL3ST2), which is expressed in cell lines with high metastatic potential. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format of the antibody was used for staining of fresh frozen human colon cancer tissue, demonstrating it could bind to individual cells and to discrete stromal components. The original format of the antibody was employed for lipid immunoblotting of a total lipid extract of LS180 cells. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |