Mfg Part Number
Ab04187-23.0
Vector Labs Ab04187-23.0 Anti-CCL11 [CAT-213 (Bertilimumab; iCo-008)], Rabbit IgG, kappa (100 μg)
Quick Overview
The original scFv antibody 3G3 was isolated from a human scFv phage display library after three rounds of panning against human eotaxin1. Optimization of 3G3 was conducted by heavy chain CDR3 randomization with point mutagenesis and light chain shuffling to obtain CAT-212 (scFv). Later on the sequence of CAT-212 was used to generate human IgG4 version of the antibody, which was called CAT-213.
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| Product Class: | Purified |
|---|---|
| Clone ID: | CAT-213 (Bertilimumab; iCo-008) |
| Synonyms: | SCYA11; Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11; 3G3 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; inhibit; neutralize |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG4 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human eotaxin (CCL11). This CC chemokine directly promotes the accumulation of eosinophils in response to the presence of allergens, which is a prominent feature of allergic inflammatory reactions. It binds to CCR3. |
| Application Notes: | The binding specificity of this antibody for eotaxin 1 was demonstrated using ELISA. The scFv version of this antibody binds human eotaxin1 with a dissociation constant (KD) of 80.4 pM. This antibody demonstrated inhibition of eotaxin1 stimulated chemotaxis of L1.2-hCCR3 cells in vitro. This antibody also inhibited eotaxin1-induced eosinophil shape change with an IC50 of 0.71 nM. In vivo administration of human eotaxin1 to the air pouch of ovalbumin-sensitized mice treated with IL-5 caused a significant dose-related increase in the recruitment of leukocytes to the pouch after 6 h (PMID: 16973884). A paper studied the contribution of eotaxin to chemotactic activity was studied by using a high-affinity neutralizing human anti-eotaxin antibody, CAT213. It was reported that this antibody significantly inhibited chemotactic activity of eosinophils in all asthmatic groups (PMID: 15001461) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |