Vector Labs Ab04197-1.1 Anti-gelatinase B [REGA-3G12], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: REGA-3G12
Synonyms: GB; GELB; MMP 9; MMP9; MMP-9; gelatinase MMP 9; 92-kDa gelatinase; matrix metalloproteinase 9; Matrix metalloproteinase-9; neutrophil gelatinase; natural human neutrophil gelatinase B; type V collagenase; 92-kDa type IV collagenase; macrophage gelatinase; 95 kDa type IV collagenase/gelatinase; collagenase IV; collagenase type IV; type IV collagen metalloproteinase; 3G12; 3G12T; 3G12 scFv; 3G12scFv
Amount: 100 μg
Application Codes Clone: ELISA; Immunoblot; Inhibition; IP; WB
Shipping Temperature: Wet Ice
Origin Pub PMID: 9323036
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for human gelatinase B; it recognizes the catalytic site region between Trp₁₁₆ and Lys₂₁₄ of the enzyme's globular aminoterminal part. A dimer scFv (with a His₆ tag) variant of this antibody was found to cross-react with gelatinase A.
Application Notes: The specificity of this antibody's original format (mouse IgG1, κ) was evaluated by ELISA; it specifically bound to gelatinase B with a binding affinity of 2.1 x 10^-9 M. This antibody detected both native and H₂NPhAcHg-treated gelatinase B in an immunoblot, both in non-reducing and reducing conditions. Furthermore, this antibody was found to inhibit gelatinase B activity in vitro (Paemen et al., 1995; PMID: 8575432). The binding of the scFv version of this antibody to gelatinase B was confirmed in an ELISA. The original antibody and its F(ab')₂ and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997; PMID: 9323036). The following scFv derivatives of this antibody were evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv with (His₆) monomer (10 μM), REGA-3G12 scFv with (His₆) dimer (5 μM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 μM). They achieved 6.5%, 65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. Moreover, the scFv His-tag dimer variant was found to cross-react with gelatinase A with a 75% inhibitory activity (same antibody concentration) (Hu et al., 2004; PMID: 15104254). The original format of the antibody was used to detect and characterize the binding of this antibody to gelatinase B via immunoprecipitation (IP) and Western blot (WB), and the catalytic domain in the aminoterminal segment of gelatinase B was identified as its target (Martens et al., 2007; PMID: 17137715).

Product Class:	Purified
Clone ID:	REGA-3G12
Synonyms:	GB; GELB; MMP 9; MMP9; MMP-9; gelatinase MMP 9; 92-kDa gelatinase; matrix metalloproteinase 9; Matrix metalloproteinase-9; neutrophil gelatinase; natural human neutrophil gelatinase B; type V collagenase; 92-kDa type IV collagenase; macrophage gelatinase; 95 kDa type IV collagenase/gelatinase; collagenase IV; collagenase type IV; type IV collagen metalloproteinase; 3G12; 3G12T; 3G12 scFv; 3G12scFv
Amount:	100 μg
Application Codes Clone:	ELISA; Immunoblot; Inhibition; IP; WB
Shipping Temperature:	Wet Ice
Origin Pub PMID:	9323036
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for human gelatinase B; it recognizes the catalytic site region between Trp₁₁₆ and Lys₂₁₄ of the enzyme's globular aminoterminal part. A dimer scFv (with a His₆ tag) variant of this antibody was found to cross-react with gelatinase A.
Application Notes:	The specificity of this antibody's original format (mouse IgG1, κ) was evaluated by ELISA; it specifically bound to gelatinase B with a binding affinity of 2.1 x 10^-9 M. This antibody detected both native and H₂NPhAcHg-treated gelatinase B in an immunoblot, both in non-reducing and reducing conditions. Furthermore, this antibody was found to inhibit gelatinase B activity in vitro (Paemen et al., 1995; PMID: 8575432). The binding of the scFv version of this antibody to gelatinase B was confirmed in an ELISA. The original antibody and its F(ab')₂ and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997; PMID: 9323036). The following scFv derivatives of this antibody were evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv with (His₆) monomer (10 μM), REGA-3G12 scFv with (His₆) dimer (5 μM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 μM). They achieved 6.5%, 65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. Moreover, the scFv His-tag dimer variant was found to cross-react with gelatinase A with a 75% inhibitory activity (same antibody concentration) (Hu et al., 2004; PMID: 15104254). The original format of the antibody was used to detect and characterize the binding of this antibody to gelatinase B via immunoprecipitation (IP) and Western blot (WB), and the catalytic domain in the aminoterminal segment of gelatinase B was identified as its target (Martens et al., 2007; PMID: 17137715).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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