Mfg Part Number
Ab04198-10.3
Vector Labs Ab04198-10.3 Anti-RSPO1 [89M5], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice against recombinant human RSPO1 protein amino acids 31-263.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 89M5 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | R-spondin-1; Roof plate-specific spondin-1; hRspo1 |
| Amount: | 100 μg |
| Application Codes Clone: | SPR; FACS; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody specifically binds human RSPO1. The antibody cross-reacts with mouse RSPO1 |
| Application Notes: | The binding affinity of the original format of the antibody to human and mouse RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM respectively). Further, the binding affinity of the humanized version of the antibody, h89M5-H2L2, to human RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM). The original format of the antibody could inhibit tumor cell growth in vivo in an ovarian tumor xenograft model as single agents and in combination with a chemotherapeutic agent. The original format of the antibody could inhibit tumor cell growth in vivo in a pancreatic tumor xenograft model in combination with a chemotherapeutic agent. The original antibody was able to block RSPO1-induced β-catenin signaling in HEK-293 cells. The original antibody blocked binding of RSPO1 to LGR5 in HEK-293 cells, as measured by FACS. The original and humanized format of the antibody, could bind to human RSPO1 expressed on HEK-293 cells, as shown by FACS analysis (US8802097B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |