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Mfg Part Number

Ab04207-10.3

Vector Labs Ab04207-10.3 Anti-Giantin [TA10], Human IgG1, Fc Silent™, lambda (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.

$570.70

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Description

Product Class: Purified
Clone ID: TA10
Heavy Chain Modification: Fc Silent™
Synonyms: Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; GCP372; Macrogolgin
Amount: 100 μg
Application Codes Clone: WB; IF; in vivo
Shipping Temperature: Wet Ice
Origin Pub PMID: 14617357
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for a 79 amino acid long region between amino acids 1462 to 1540 of the full human sequence (corresponding to amino acids 1399 to 1478 in the rat homologue) in Giantin, a Golgi matrix protein.
Application Notes: The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence straining of Giantin in HeLa cells. siRNA knock-down of Giantin reduced TA10 staining, confirming its specificity. Furthermore, an in vivo expressed TA10-GFP construct successfully stained HeLa cells (Nizak et al., 2003; PMID: 14617357). The scFv-Fc fusion version of this antibody — hTA10 — detected Giantin when cells were fixed by paraformaldehyde (Moutel et al., 2009; PMID: 19245715). Precise characterization of the TA10 target was achieved; a region of 79 amino acids, representing less than 2.5% of the entire protein, was determined as its binding site (Vielemeyer et al., 2010; PMID: 20727208).

Product Class:	Purified
Clone ID:	TA10
Heavy Chain Modification:	Fc Silent™
Synonyms:	Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; GCP372; Macrogolgin
Amount:	100 μg
Application Codes Clone:	WB; IF; in vivo
Shipping Temperature:	Wet Ice
Origin Pub PMID:	14617357
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for a 79 amino acid long region between amino acids 1462 to 1540 of the full human sequence (corresponding to amino acids 1399 to 1478 in the rat homologue) in Giantin, a Golgi matrix protein.
Application Notes:	The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence straining of Giantin in HeLa cells. siRNA knock-down of Giantin reduced TA10 staining, confirming its specificity. Furthermore, an in vivo expressed TA10-GFP construct successfully stained HeLa cells (Nizak et al., 2003; PMID: 14617357). The scFv-Fc fusion version of this antibody — hTA10 — detected Giantin when cells were fixed by paraformaldehyde (Moutel et al., 2009; PMID: 19245715). Precise characterization of the TA10 target was achieved; a region of 79 amino acids, representing less than 2.5% of the entire protein, was determined as its binding site (Vielemeyer et al., 2010; PMID: 20727208).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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