Mfg Part Number
Ab04209-10.3
Vector Labs Ab04209-10.3 Anti-Non muscle Myosin IIA [SF9], Human IgG1, Fc Silent™, lambda (100 μg)
Quick Overview
The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | SF9 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | nmMyoIIA; NMMHC II-a; NMMHC-IIA; NMMHC-A; Non-muscle myosin heavy chain Iia; Myosin-9; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle Iia; Non-muscle myosin heavy chain A |
| Amount: | 100 μg |
| Application Codes Clone: | WB; IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 14617357 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for a very small (29 aa long; <3 kDa) epitope of non muscle Myosin IIA, starting at aa 1536 in the full human sequence. |
| Application Notes: | The original format of this antibody (scFv) was successfully used in a Western blot and in for in vitro immunofluorescence staining of non muscle Myosin IIA (nmMyoIIA) in HeLa cells (Nizak et al., 2003; PMID: 14617357). Precise characterization of the SF9 target was achieved, defining a very small (<3 kDa) epitope within the ~200kDa nmMyoIIA protein, revealing cross-reactivity with closely related proteins of the same and across species (Vielemeyer et al., 2010; PMID: 20727208). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |