Vector Labs Ab04211-1.159 Anti-Tenuazonic acid [3F9], Mouse IgG1-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3F9
Synonyms: Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)-butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one
Amount: 100 μg
Application Codes Clone: ELISA; chemiluminescent enzyme immunoassay; bioluminescent enzyme immunoassay
Shipping Temperature: Wet Ice
Origin Pub PMID: 32702970
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Dromedary VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN.
Application Notes: The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC₅₀ value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.

Product Class:	Purified
Clone ID:	3F9
Synonyms:	Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)-butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one
Amount:	100 μg
Application Codes Clone:	ELISA; chemiluminescent enzyme immunoassay; bioluminescent enzyme immunoassay
Shipping Temperature:	Wet Ice
Origin Pub PMID:	32702970
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Dromedary VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN.
Application Notes:	The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC₅₀ value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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