Mfg Part Number
Ab04214-10.0
Vector Labs Ab04214-10.0 Anti-Amyloid beta [scFv59], Human IgG1, lambda (100 μg)
Quick Overview
The original antibody was isolated from a human scFv phage library by screening against Abeta1-42.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | scFv59 |
| Synonyms: | Aβ; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); PreA4; Protease nexin-II (PN-II) |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IHC; WB; ThT assay; MTT assay; in vivo |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 16630540 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for oligomeric as well as fibrillar/aggregated Aβ. |
| Application Notes: | The scFv version of this antibody demonstrated the highest ELISA titer for Aβimmunoreactivity against Aβ1–42, and its specificity for oligomeric and fibrillar/aggregated Aβ was demonstrated in a WB. The scFv version of this antibody reacted with amyloid beta deposits in brain sections from a Tg2576 mouse and in skeletal muscle sections from a Tg13592 mouse, as observed via IHC. The scFv version of this antibody was used to inhibit the formation of Aβ fibril formation in vitro as demonstrated in a Thioflavin T assay. This inhibition contributed to the amelioration of Aβ cytotoxicity as was shown in vitro in an MTT assay; cell viability increased from 45.2 ± 0.78% to 58.8 ± 5.45% (P = 0.069) in the presence of scFv59. Furthermore, Tg2576 mice subjected to scFv59 injection in the hippocampus and cortex had fewer diffuse and fibrillar Aβ deposits in those areas than Tg2576 mice injected with PBS (Fukuchi et al., 2006; PMID: 16630540). Vascular amyloid was also stained by scFv59 (Fukuchi et al., 2006; PMID: 16766200). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |