Mfg Part Number
Ab04233-34.11-BS

Vector Labs Ab04233-34.11-BS Anti-HCEC [FC44], Camelid VHH, His-tagged, (1 mg)

Quick Overview
The original antibody was isolated from a naive phage displayed library of llama VHH. The library was panned against HCEC target cells.
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Product Class:Purified
Clone ID:FC44
Heavy Chain Modification:His-tagged
Synonyms:Human cerebromicrovascular endothelial cells
Amount:1 mg
Application Codes Clone:ELISA; in vivo; in vitro
Shipping Temperature:Wet Ice
Origin Pub PMID:11772942
Buffer Composition:PBS only.
Original Format:VHH
Storage Temperature:Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:The antibody is specific for HCEC.
Application Notes:The specificity of the original format of the antibody was confirmed by ELISA analysis (US8715659B2; Muruganandam et al., 2002; PMID: 11772942). The original format of the antibody bound to the vesicular and the membrane fractions of HCEC, and it was taken up by living HCEC, as shown by fluorescence microscopy. The antibody could transmigrate across an in vitro human blood-brain barrier (BBB) model. The antibody was also also detected in the brain after i.v. injection into mice (Muruganandam et al., 2002; PMID: 11772942). The antibody was able to transport across rat BBB, as demonstrated by in vitro and in vivo studies (Xiao et al., 2013; PMID: 23840214).
More Information
Brand Vector Labs
UNSPSC 12161500