Mfg Part Number
Ab04234-30.11
Vector Labs Ab04234-30.11 Anti-KRAS G12V [G12V-34], scFv fragment (His), ScFv, (100 μg)
Quick Overview
The original antibody was generated through the construction of a phage-displayed single-chain fragment variable (scFv) library from mice splenocytes immunized with specific mimotope peptides. The library was subjected to three rounds of biopanning on G12V and G13D peptide-coated plates.
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| Product Class: | Purified |
|---|---|
| Clone ID: | G12V-34 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | Mutated KRAS G12V; Mutated Kirsten rat sarcoma viral oncogene homolog G12V; Kirsten Rat Sarcoma virus; Kirsten Rat Sarcoma virus; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; K-ras; KRAS proto-oncogene; GTPase; c-Ki-ras2; OES; c-Ki-ras; K-Ras 2; 'C-K-RAS; K-Ras |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; MTT cytotoxicity assay; IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 33959410 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for mutated KRAS G12V. |
| Application Notes: | The original format of this antibody (scFv) was screened against WT and mutated G12 and G13 KRAS and shown to specifically bind mKRAS G12V in an ELISA. The scFv format of this antibody was fused to mHALT-1 toxin and exhibited cytotoxic effects on SW-480 colorectal cancer cells with IC50 of 25.39 μg/mL, with minimal cytotoxicity effect on NHDF cells. The scFv format of this antibody was fused to enhanced GFP and used for IF (Teo et al., 2021; PMID: 33959410). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |