Vector Labs Ab04237-1.1 Anti-IL-17 [Antibody 7], Mouse IgG1, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Antibody 7
Synonyms: IL17; IL-17A; IL17A; CTLA8; Interleukin-17A; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; IL17F; IL-17F; Interleukin-17F; IL-17F; Cytokine ML-1
Amount: 100 μg
Application Codes Clone: ELISA; SPR; functional assays; in vivo; Crystallization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for IL-17; it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature IL-17 sequence. In humans, It has been shown to bind both IL-17A and IL-17F.
Application Notes: The IC₅₀ values for this antibody as measured in an HT1080 assay in response to 1 nM human or cynomolgus monkey (cyno) IL-17A were 0.8 ± 0.2 nM and 4.8 ± 1.6 nM, respectively. Partial inhibition was also observed with IL-17F. Further assays showed cross-reactivity with dog IL-17A. This antibody neutralized the synergistic IL-6 response as measured in an altered human HT1080 assay with an IC₅₀ value of 0.24 ± 0.05 nM. pA₂ = 9.23 and Kd = 663 pM were measured when testing the affinity of this antibody for human IL-17A. Further kinetic analysis on recombinant human IL-17A (E. coli), cyno IL-17A (E. coli), and human IL-17A-Flag-His10 (HEK) yielded a 1:1 Langmuir K_D and Bivalent Analyte K_D of 0.195 nM, 0.512 nM, and 0.171 nM, and 2.098 nM, 1.340 nM, and 0.41-1.88 nM, appropriately. The biological activity of this antibody was tested extensively in the air pouch of Balb/c; it effectively inhibited IL-17A-induced IL-6 release and the influx of white blood cells in the air pouch, as reflected by low ID₅₀ values. This antibody exhibited inhibitory effects on the IL-17A/TNFα synergized responses in post-mortem cartilage explants and synovial fibroblasts with the potential to modulate inflammatory and destructive responses induced by the synergistic action of IL-17A and TNFα. X-ray crystallography of the IL-17A bound Fab version of this antibody indicated that it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature sequence (US8865166).

Product Class:	Purified
Clone ID:	Antibody 7
Synonyms:	IL17; IL-17A; IL17A; CTLA8; Interleukin-17A; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; IL17F; IL-17F; Interleukin-17F; IL-17F; Cytokine ML-1
Amount:	100 μg
Application Codes Clone:	ELISA; SPR; functional assays; in vivo; Crystallization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for IL-17; it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature IL-17 sequence. In humans, It has been shown to bind both IL-17A and IL-17F.
Application Notes:	The IC₅₀ values for this antibody as measured in an HT1080 assay in response to 1 nM human or cynomolgus monkey (cyno) IL-17A were 0.8 ± 0.2 nM and 4.8 ± 1.6 nM, respectively. Partial inhibition was also observed with IL-17F. Further assays showed cross-reactivity with dog IL-17A. This antibody neutralized the synergistic IL-6 response as measured in an altered human HT1080 assay with an IC₅₀ value of 0.24 ± 0.05 nM. pA₂ = 9.23 and Kd = 663 pM were measured when testing the affinity of this antibody for human IL-17A. Further kinetic analysis on recombinant human IL-17A (E. coli), cyno IL-17A (E. coli), and human IL-17A-Flag-His10 (HEK) yielded a 1:1 Langmuir K_D and Bivalent Analyte K_D of 0.195 nM, 0.512 nM, and 0.171 nM, and 2.098 nM, 1.340 nM, and 0.41-1.88 nM, appropriately. The biological activity of this antibody was tested extensively in the air pouch of Balb/c; it effectively inhibited IL-17A-induced IL-6 release and the influx of white blood cells in the air pouch, as reflected by low ID₅₀ values. This antibody exhibited inhibitory effects on the IL-17A/TNFα synergized responses in post-mortem cartilage explants and synovial fibroblasts with the potential to modulate inflammatory and destructive responses induced by the synergistic action of IL-17A and TNFα. X-ray crystallography of the IL-17A bound Fab version of this antibody indicated that it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature sequence (US8865166).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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