Mfg Part Number
Ab04239-19.0
Vector Labs Ab04239-19.0 Anti-Collagen I [AFA], Mouse IgA, kappa (50 μg)
Quick Overview
The original antibody was produced by immunizing mice with a synthetic peptide (GGGYDFGYDGDFYRA) whose sequence corresponds to that of the C-terminal telopeptide of the α2 chain (α2Ct).
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| Product Class: | Purified |
|---|---|
| Clone ID: | AFA |
| Synonyms: | CTTR1; α2Ct; α2(I)C-telopeptide; C-terminal telopeptide of the α2 chain; Procollagen I |
| Amount: | 50 μg |
| Application Codes Clone: | block; in vivo; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgA |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for human collagen I, showing high affinity to the GDF sequence (in A2_DGDFY, part of the α2(I)C-telopeptide), suggesting it is a critical epitope. |
| Application Notes: | This antibody binds human collagen I and blocks the binding activity of its C-terminal telopeptide region (denoted as CTTR1) consisting of two α1(I)C-telopeptides (denoted as α1Ct) and one α2(I)C-telopeptide (denoted as α2Ct). The KD of this antibody and its Fab and scFv with native α2Ct present in procollagen I and with synthetic α2Ct were 663 pM, 268 nM, 21 pM, 57 nM, and 75nM for full-length AFA/procollagen I, Fab AFA antibody/procollagen I, full-length AFA antibody/synthetic α2Ct, Fab AFA antibody/synthetic α2Ct, and scFv/procollagen I, respectively (US20200048333A1). A chimeric IgG version of the original IgA version of this antibody was engineered (Fertala et al., 2013; PMID: 23586407). This antibody has been shown to be an effective therapeutic tool for blocking collagen fibril formation in various models (Fertala et al., 2017; PMID: 28972447) (Fertala et al., 2017; PMID: 23586407). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |