Mfg Part Number
Ab04247-1.1

Vector Labs Ab04247-1.1 Anti-CD50 [101-1D2], Mouse IgG1, kappa (100 μg)

Quick Overview
The original antibody was raised by immunizing mice with a mixed leukocyte culture.
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Product Class:Purified
Clone ID:101-1D2
Synonyms:ICAM3; CDw50; ICAM-R; ICAM-3
Amount:100 μg
Application Codes Clone:IF; FC; inhibition; IP; IHC; WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody recognizes CD50 domain 1.
Application Notes:The original version of this antibody was used for immunofluorescence on T cells, B cells and non lymphoic cell lines (Juan et al., 1993; PMID:8325327), on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), and on peripheral blood stimulated or unstimulated cells . Furthermore this antibody was used for flow cytometry on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), on peripheral blood stimulated or unstimulated cells (Vilella et al., 1990; PMID:1710077), and on T cells (Vilardell et al., 1998; PMID: 9672149). This antibody was also used for immunoprecipitation on PMBCs to determine whether CD50 and ICAM-3 are the same protein (Juan et al., 1993; PMID:8325327), and on peripheral blood stimulated or unstimulated cells to prove that 101–1D2 and 140–11 recognize the same molecule (Vilella et al., 1990; PMID:1710077). This antibody was used for immunohistochemistry on human neoplasms to study the expression of CD50 (Terol et al., 1996; PMID: 8946680). The ability of this antibody to inhibit cell-mediated lympholysis was tested in an inhibition assay on PMBCs was tested, it showed it was able to inhibit lympholysis (Vilella et al., 1990; PMID:1710077). Finally this antibody was used for western blot on immunoprecipitated proteins. This experiment shows that the CD50 protein coprecipitates with a 56-kD lck protein and a 59-kD fyn PTK (Juan et al., 1994; PMID:7515097).
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Brand Vector Labs
UNSPSC 12161500