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Mfg Part Number

Ab04257-10.3

Vector Labs Ab04257-10.3 Anti-MUC-5AC [2-12M1], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing BALB/c mice with preparations of M1 antigens isolated from ovarian mucinous fluid of a pure endocervical type from an ALe(a-b+) patient.

$570.70

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Description

Product Class: Purified
Clone ID: 2-12M1
Heavy Chain Modification: Fc Silent™
Synonyms: Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
Amount: 100 μg
Application Codes Clone: WB; ICC; ELISA; IHC; IF; IRA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the C-terminus of MUC-5AC.
Application Notes: The original version of this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein (Lidell et al., 2008; PMID:18167142), on mucin proteins obtained from whole human stomachs (Mall et al., 1999; PMID:10343135), and on mucin proteins (Bara et al., 1988; PMID:2460087). This antibody was also used to stain MUC5AC in immunofluorescence on COS-7 cells transfected with pSecTag2 carrying fragments of the 59 end cDNA of MUC5AC (Nollet et al., 2004; PMID:15165482), and on BxPC-3, HT-29, LS174T, MCF-7, Calu-3 and Capan-1 cells (Liu et al., 2015; PMID:25595893). Furthermore this antibody was also used for immunoradiometric staining on fractions obtained during protein purification (Bara et al., 1991; PMID:1988372), and on a colon mucin -preparation of an 0 Le(a-b-) patient (Bara et al., 1988; PMID:2460087). Additionally this antibody was used for an ELISA on a M1 antigen preparation. And finally, this antibody was used for immunohistochemistry to stain the M1 antigen in fetal colonic mucosae (Bara et al., 1986; PMID:3524800).

Product Class:	Purified
Clone ID:	2-12M1
Heavy Chain Modification:	Fc Silent™
Synonyms:	Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
Amount:	100 μg
Application Codes Clone:	WB; ICC; ELISA; IHC; IF; IRA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the C-terminus of MUC-5AC.
Application Notes:	The original version of this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein (Lidell et al., 2008; PMID:18167142), on mucin proteins obtained from whole human stomachs (Mall et al., 1999; PMID:10343135), and on mucin proteins (Bara et al., 1988; PMID:2460087). This antibody was also used to stain MUC5AC in immunofluorescence on COS-7 cells transfected with pSecTag2 carrying fragments of the 59 end cDNA of MUC5AC (Nollet et al., 2004; PMID:15165482), and on BxPC-3, HT-29, LS174T, MCF-7, Calu-3 and Capan-1 cells (Liu et al., 2015; PMID:25595893). Furthermore this antibody was also used for immunoradiometric staining on fractions obtained during protein purification (Bara et al., 1991; PMID:1988372), and on a colon mucin -preparation of an 0 Le(a-b-) patient (Bara et al., 1988; PMID:2460087). Additionally this antibody was used for an ELISA on a M1 antigen preparation. And finally, this antibody was used for immunohistochemistry to stain the M1 antigen in fetal colonic mucosae (Bara et al., 1986; PMID:3524800).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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