Vector Labs Ab04261-10.0 Anti-MUC-5AC [4-5M1], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 4-5M1
Synonyms: 45M1; Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
Amount: 100 μg
Application Codes Clone: IF; IP; WB; ELISA; IHC; ICC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This body is specific for the 5' terminal epitope of the MUC5AC.
Application Notes: The original version of this antibody was used for ELISA on NCI-H292 human pulmonary mucoepidermoid cells (Baginski et al., 2006; PMID:16543607), on BAL fluid (bronchoalveolar lavage fluid) (Phillips et al., 2006; PMID:16507438), and on different fractions of corpus tissue (Nordman et al., 2002; PMID:11988092). This antibody was also used for flow cytometry on human lung cells (Weeden et al., 2017; PMID:28125611), and on H292 cells (Montalbano et al., 2014; PMID:24797786). It was able to stain MUC5AC in both experiments. This antibody was able to stain passage 2 normal human tracheobronchial epithelial (NHTBE) cells for immunofluorescence, it was able to determine MUC5AC production during this experiment (Gray et al., 2004; PMID:14527933). The antibody was also used to stain HT29–18N2 goblet cells in a immunocytochemistry experiment, showing staining of the intracellular mucin stores (Stanley and Phillips., 1999; PMID:10409167). Immunoprecipitation on a mucin solution was performed using this antibody, allowing MUC5AC to be separated from the solution (Round et al., 2007; PMID:17356062). Finally, this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein. showing that it bound specifically to the C-terminus of MUC-5AC (Lidell et al., 2008; PMID:18167142).

Product Class:	Purified
Clone ID:	4-5M1
Synonyms:	45M1; Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
Amount:	100 μg
Application Codes Clone:	IF; IP; WB; ELISA; IHC; ICC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This body is specific for the 5' terminal epitope of the MUC5AC.
Application Notes:	The original version of this antibody was used for ELISA on NCI-H292 human pulmonary mucoepidermoid cells (Baginski et al., 2006; PMID:16543607), on BAL fluid (bronchoalveolar lavage fluid) (Phillips et al., 2006; PMID:16507438), and on different fractions of corpus tissue (Nordman et al., 2002; PMID:11988092). This antibody was also used for flow cytometry on human lung cells (Weeden et al., 2017; PMID:28125611), and on H292 cells (Montalbano et al., 2014; PMID:24797786). It was able to stain MUC5AC in both experiments. This antibody was able to stain passage 2 normal human tracheobronchial epithelial (NHTBE) cells for immunofluorescence, it was able to determine MUC5AC production during this experiment (Gray et al., 2004; PMID:14527933). The antibody was also used to stain HT29–18N2 goblet cells in a immunocytochemistry experiment, showing staining of the intracellular mucin stores (Stanley and Phillips., 1999; PMID:10409167). Immunoprecipitation on a mucin solution was performed using this antibody, allowing MUC5AC to be separated from the solution (Round et al., 2007; PMID:17356062). Finally, this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein. showing that it bound specifically to the C-terminus of MUC-5AC (Lidell et al., 2008; PMID:18167142).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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