Mfg Part Number
Ab04276-10.3
Vector Labs Ab04276-10.3 Anti-CK18 (Cytokeratin 18) [C-04], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with keratin-enriched preparations from cultured A431 cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | C-04 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | KRT18; K18; CK-18; Keratin-18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18 |
| Amount: | 100 μg |
| Application Codes Clone: | IHC; IF; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for CK-18. |
| Application Notes: | The original version of this antibody was used for immunohistochemistry on sections of teratoma derived from iPS cells (Shao et al., 2009; PMID:19238173), on rat endometrial cell sheets and the resected uteri (Kuramoto et al., 2018; PMID:29980256), on fresh samples from either tumours or normal tissues distant from tumours (Bártek et al., 1991; PMID:1716305), and on sebaceous epithelioma tissue from 2 dogs (Yoon and Park., 2016; PMID:27822240). To determine the specificity of this antibody immunofluorescence was performed on A431 cells and the HeLa cytoskeleton. Further a western blot was performed on filament-enriched preparations using this antibody (Bártek et al., 1991; PMID:1716305). This antibody was also used for immunofluorescence on Fresh IT specimens in a study on nose polyps (Yu et al., 2013; PMID:24033346). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |