Mfg Part Number
Ab04277-10.3

Vector Labs Ab04277-10.3 Anti-Pan-cytokeratin [C-11], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with keratin-enriched preparations from cultured A431 cells.
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Product Class:Purified
Clone ID:C-11
Heavy Chain Modification:Fc Silent™
Amount:100 μg
Application Codes Clone:ICC; IHC; IF; WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for cytokeratin.
Application Notes:The original version of this antibody was used for immunocytochemistry on endometrial tissue from whole uteri of regularly cycling women (Ciocca et al., 1983; PMID: 6348061). This antibody was also used for immunohistochemistry on CIS from a DMBA-treated rat (Heffelfinger et al., 2003; PMID: 12861040), on histological sections of CIS from untreated and 6-week ZD6474 cohorts (Heffelfinger et al., 2004; PMID: 15170218), on mouse thymus (Song et al., 2008; PMID: 18824586), and on fresh samples from either tumors or normal tissues distant from tumors (Bártek et al., 1991; PMID: 1716305). This antibody was also used for immunofluorescence on Skin pieces of juvenile goldfish (Mauger et al., 2009; PMID: 19068235). This antibody was used in a western blot on mouse intestine lysates (Rhee et al., 2009; PMID: 19188353), on K562 and MDAMB231 cell lines (Kershaw et al., 2015; PMID: 25957999), and on filament-enriched preparations using this antibody (Bártek et al., 1991; PMID: 1716305).
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Brand Vector Labs
UNSPSC 12161500