Mfg Part Number
Ab04287-10.3

Vector Labs Ab04287-10.3 Anti-CK18 (Cytokeratin 18) [DC10], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
The original antibody was raised by immunizing mice with human breast cancer-derived cell line PMC 42.
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Product Class:Purified
Clone ID:DC10
Heavy Chain Modification:Fc Silent™
Synonyms:KRT18; K18; CK-18; Keratin-18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
Amount:100 μg
Application Codes Clone:IHC; WB; ELISA; IP; FC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for cytokeratin 18
Application Notes:The original version of this antibody was used for a western blot gel-separated extracts fromvarious cultured cells (Lauerová et al., 1988; PMID:2461901). This antibody was used for immunofluorescence on various human cultured cells (Lauerová et al., 1988; PMID:2461901). This antibody was also used for immunohistochemistry on human kidney and urethra (Lauerová et al., 1988; PMID:2461901), and on epithelial cell rests of Malassez (Rincon et al., 2006; PMID:16827716), and on specimens from nontumor liver diseases and hepatocellular carcinomas (Pei et al., 2004; PMID:15221653). This antibody was used for an ELISA on cytokeratins (Tao et al., 2008; PMID:18393369). This antibody was used for immunoprecipitation on BHK-21 (baby hamster kidney), NIH3T3 (mouse fibroblast), HepG2 (human hepatoma), and HT29 cells and on liver tissues (Snider et al., 2013; PMID:23358244). Furthermore this antibody was used for flow cytometry on hES cells (Metallo et al., 2011; PMID:19907998).
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Brand Vector Labs
UNSPSC 12161500