Mfg Part Number
Ab04311-10.3
Vector Labs Ab04311-10.3 Anti-Tubulin [TU-02], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was raised by immunzing a mosue with tubulin isolated from pig brain.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | TU-02 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TUBA3; TUBA1A; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; Tubulin alpha-1A chain |
| Amount: | 100 μg |
| Application Codes Clone: | WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for alpha tubulin. |
| Application Notes: | The original antibody was used for a western blot on A549-ACE2 cells treated with CBD. To determine effect of CBD on SARS-Covid 2 infections (Nguyen et al., 2022; PMID:35050692). This antibody was used for a western blot on human TNBC cell lines BM1 and MB436, and mouse TNBC cell lines LMB and M6C (Yesilkanal et al., 2021; PMID:33973518), on total protein lysate from NHP podocytes (Zheng et al., 2018; PMID:30429458), and on total extract of porcine brain (Dráber et al., 1989; PMID:2480356). The original format of the antibody was successfully used in immunofluorescence in different tissues, such the granular layer of the skin, nerves and the superficial cells of the epithelium of the trachea (Dráber et al., 1987; PMID:2442125), and on cytoskeletons extracted from 3T3-Swiss albino fibroblast-like cells from mouse embryos (Dráber et al., 1989; PMID:2480356). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |