Mfg Part Number
Ab04359-10.3
Vector Labs Ab04359-10.3 Anti-CD71 [ch128.1], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The antibody was constructed by substituting the variable regions of anti-dansyl IgG3 with those of the murine IgG1 anti-human TfR monoclonal Ab (mAb) 128.1. Ab128.1 was generated by immunizing mice with the external domain of the human transferrin (Tf) receptor.
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| Product Class: | Purified |
|---|---|
| Clone ID: | ch128.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TFRC; TfR1; TR; TfR; hTfR1; Transferrin receptor protein 1; p90; 128.1; anti-hTfR IgG3 |
| Amount: | 100 μg |
| Application Codes Clone: | in vitro; in vivo; cristallography |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody binds the apical domain of hTfR1. |
| Application Notes: | The IgG3 format of the antibody cross-linked with a secondary antibody inhibits the growth and induces apoptosis in ARH-77 cells. The IgG3 format of the antibody cross-linked with a secondary Ab induces intracellular degradation of the TfR (Ng et al., 2006; PMID: 16804109). The IgG3 format of the antibody was able to block the entry of MLV pseudotyped with the envelope glycoproteins of JUNV, GTOV, CHAV, SABV, and MACV in CHO-K1 cells. The 50% inhibitory concentration (IC50) of the IgG3 format of the antibody for these pseudoviruses was measured in HEK293T cells. The antibody showed subnanomolar IC50 for all NW hemorrhagic fever arenaviruses. The IgG3 format of the antibody could antagonize replication-competent JUNV. Surface plasmon resonance assays confirmed that the IgG3 format of the antibody has a high affinity for hTfR1 (Kd = 5.7 nM). The IgG3 format of the antibody detected CD71 in different kind of cells (HEK 293T, sMAGI, Vero) by flow cytometry. The antibody was able to block JUNV pseudovirus entry in sMAGI and Vero cells (Helguera et al., 2012; PMID: 22278244). The IgG1 format of the antibody could inhibit JUNV infection in vitro (EC90 = 0.73 nM). The IgG1 format of the antibody protects hTfR1-expressing transgenic mice against lethal NWM challenge. The crystal structure of the Fab fragment of the antibody was determined (Hickerson et al., 2022; PMID: 35091550). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |