Mfg Part Number
Ab04384-23.0
Vector Labs Ab04384-23.0 Anti-Capsid protein VP1 [Mab14], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with purified Capsids of CPV and FPV which were prepared from infected feline cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Mab14 |
| Synonyms: | Coat protein VP1; CPV-2; Fab 14; Antibody 14; ABCD_AD535 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; NTRL; HI; RIA; cryoEM; Crystallography |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 19321620 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Site A of CPV-2's capsid. |
| Application Notes: | The original version of this antibody (IgG2a) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV-a and -b in neutralization and HI assays (Parrish et al., 1982; PMID: 6180709). The original version of this antibody was used in RIA and HI assays. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, and in the RIA, it was found to react with CPV-d variants 6, 12, and 8 (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was measured via ELISA as 3.4 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |