Mfg Part Number
Ab04386-23.0
Vector Labs Ab04386-23.0 Anti-Capsid protein VP1 [MabB], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing a Lewis-strain rat with purified infectious (heavy) FPV-b.
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| Product Class: | Purified |
|---|---|
| Clone ID: | MabB |
| Synonyms: | Coat protein VP1; CPV-2; FPV; MEV; RPV; ABCD_AD539 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; NTRL; HI; RIA; Crystallography |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 19321620 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for CPV-2's, FPV's capsid (site A). It also cross-reacts with MEV and RPV. |
| Application Notes: | The original version of this antibody (rat IgG2b) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV, FPV, and MEV-a and -b in neutralization and HI assays. It was also used in RIAs. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, FPV-a; -b; -c, RPV-a; -b; -c; -d; and -e, and MEV-a; -b; -c; -d; -e; -f; -g; and -h. In the RIA, it was found to react with CPV-d and FPV-b (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was estimated via ELISA as ~6.1 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |