Mfg Part Number
Ab04432-1.29
Vector Labs Ab04432-1.29 Anti-Capsid protein [A6.2], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a A MNV-1-seropositive 129 mouse with brain homogenate containing MNV-1.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A6.2 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | ORF2CP; VP1; Major capsid protein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; neutralization; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the outermost tip of the closed conformation of the MNV-1 P2 domain. |
| Application Notes: | The original antibody was used for an neutralization assay on the RAW 264.7 cells with the P-domain of MNV-1 (Taube et al., 2010; PMID:20335262). Further a plaque neutralization assay was performed on either MNV-1.CW1* or MNV-1CW1*.fab2 (Taube et al., 2010; PMID:20335262), on MNV-1 (Kolawole et al., 2014; PMID:24501415), and on MNV-1.CW1 or MNV-1 brain homogenate (Wobus et al., 2004; PMID:15562321). The structure of the original antibody was determined using cryo-EM (Taube et al., 2010; PMID:20335262). This antibody was used for an ELISA on CsCl-purified MNV-1 particles (Wobus et al., 2004; PMID:15562321). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |