Mfg Part Number
Ab04445-10.29

Vector Labs Ab04445-10.29 Anti- Fusion glycoprotein F0 [PIA174], Human Fab fragment, His-Tagged, kappa (100 μg)

Quick Overview
The original antibody was raised by isolating it from a B cell culture supernatant.
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Product Class:Purified
Clone ID:PIA174
Heavy Chain Modification:His-Tagged
Synonyms:F; Protein F; Human respirovirus 3
Amount:100 μg
Application Codes Clone:ELISA; in vitro; neutralization; SPR
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:Fab
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for DIII domain of fusion glycoprotein F0 from human respirovirus 3.
Application Notes:The original antibody was used for an ELISA on parainfluenza virus type 3. Further the structure of this antibody was determined using cryo-EM (Stewart-Jones et al., 2018;PMID:30420505). This antibody was used for a plaque reduction assay on Vero cells infected with parental or escape variant of human respirovirus. This antibody was used for a neutralization assay. It showed here that it disrupted the formation of the HN/F complex (Marcink et al., 2023; PMID:36763666). This antibody was used for surface plasmon resonance on LuS-N71-SpyLinked-PIV3 F (Zhang et al., 2020; PMID:33097791).
More Information
Brand Vector Labs
UNSPSC 12161500