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Mfg Part Number

Ab04451-1.1

Vector Labs Ab04451-1.1 Anti-Genome polyprotein [AM28], Mouse IgG1, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised from human peripheral blood from healthy donors.

$570.70

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Description

Product Class: Purified
Clone ID: AM28
Synonyms: P1AB; P1C; P1D; P2A; P2B; Protein 2A; Protein 2B; Protein 2C; Capsid protein VP0; Capsid protein VP1; Capsid protein VP3; Virion protein 0; Virion protein 1; Virion protein 3; Human parechovirus 1
Amount: 100 μg
Application Codes Clone: ELISA; neutralization; IF; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for epitopes in VP0 and VP3.
Application Notes: The original antibody was used for a virus neutralization assay with HPeV1 and HT29 cells (Westerhuis et al., 2015; PMID:25948742, Shakeel et al., 2015; PMID:26157123). This antibody was used for an ELISA on purified HPeV1 to -6 or the capsid proteins VP0, VP3, and VP1 from HPeV1 to -4 (Westerhuis et al., 2015; PMID:25948742), and on N-terminally biotin-labelled HPeV1 overlapping peptides (Shakeel et al., 2015; PMID:26157123). This antibody was used for immunofluorescence on infected and uninfected HT29 cells. The binding affinity of this antibody was determined using surface plasmon resonance on VP1 of HPeV1 (Westerhuis et al., 2015; PMID:25948742). The structure of the original antibody was determined using cryo-EM (Domanska et al., 2019; PMID:30463974). This antibody was used as a control in a binding assay on HT29 cells with HPeV3 (Domanska et al., 2019; PMID:30463974).

Product Class:	Purified
Clone ID:	AM28
Synonyms:	P1AB; P1C; P1D; P2A; P2B; Protein 2A; Protein 2B; Protein 2C; Capsid protein VP0; Capsid protein VP1; Capsid protein VP3; Virion protein 0; Virion protein 1; Virion protein 3; Human parechovirus 1
Amount:	100 μg
Application Codes Clone:	ELISA; neutralization; IF; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for epitopes in VP0 and VP3.
Application Notes:	The original antibody was used for a virus neutralization assay with HPeV1 and HT29 cells (Westerhuis et al., 2015; PMID:25948742, Shakeel et al., 2015; PMID:26157123). This antibody was used for an ELISA on purified HPeV1 to -6 or the capsid proteins VP0, VP3, and VP1 from HPeV1 to -4 (Westerhuis et al., 2015; PMID:25948742), and on N-terminally biotin-labelled HPeV1 overlapping peptides (Shakeel et al., 2015; PMID:26157123). This antibody was used for immunofluorescence on infected and uninfected HT29 cells. The binding affinity of this antibody was determined using surface plasmon resonance on VP1 of HPeV1 (Westerhuis et al., 2015; PMID:25948742). The structure of the original antibody was determined using cryo-EM (Domanska et al., 2019; PMID:30463974). This antibody was used as a control in a binding assay on HT29 cells with HPeV3 (Domanska et al., 2019; PMID:30463974).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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