Mfg Part Number
Ab04452-23.0

Vector Labs Ab04452-23.0 Anti-Envelope glycoprotein H; Envelope glycoprotein L [Fab-RC], Rabbit IgG, kappa (100 μg)

Quick Overview
The original antibody was raised from the white blood cells of a human individual immunized with zoster vaccine.
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Product Class:Purified
Clone ID:Fab-RC
Synonyms:gH; gL; ORF60; ORF37
Amount:100 μg
Application Codes Clone:SPR; IHC; IP; crystallography; neutralization
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the combination of gH and gL and does not detect the proteins in isolation.
Application Notes:The original antibody was used for surface plasmon resonance on VZV gHgL. This antibody was used for a neutralization assay. Here is showed it was able to inhibit gB/gHgL-mediated membrane fusion. The structure of this antibody was determined using x-ray crystallography (Xing et al., 2015; PMID:25918416). This antibody was used for immunohistochemistry on fixed and unfixed VZV-infected and control HFL cells. This antibody was also used for a plaque reduction assay on VZV-infected HFL cells. Further this antibody was used for immunoprecipitation on VZV-infected and uninfected HFL cells (Birlea et al., 2013; PMID:23077312).
More Information
Brand Vector Labs
UNSPSC 12161500