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Mfg Part Number

Ab04453-10.0

Vector Labs Ab04453-10.0 Anti-Envelopment polyprotein [R12], Human IgG1, lambda (100 μg)

VECTOR LABS

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Quick Overview

This original antibody was raised by screening B cells from a Rift Valley fever patient and sequencing the VH and VL antibody regions.

$570.70

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Description

Product Class: Purified
Clone ID: R12
Synonyms: GP; M polyprotein
Amount: 100 μg
Application Codes Clone: ELISA; WB; FC; neutralizaiton; SPR; crystallography; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for the DI domain, including residues K164, H166, 173-TQEDA-177, K180, P273, K274, 291-SLK-293, and K395 of rift valley fever glycoprotein N
Application Notes: The original version of this antibody was used for an ELISA on rift valley fever Gn and Gc. Further this antibody was used for flow cytometry on HEK293T cells transiently transfected with plasmids of pEGFP-M, pEGFP-Gn, pEGFP-Gc or pEGFP-Gn mutants. This antibody was used for a blocking assay, the rift valley virus was preincubated with this antibody and the mixture was then added to Vero cells. This antibody was used for a western blot on its linear epitope. This antibody was used for surface plasmon resonance on rift valley fever Gn and Gc. A neutralization assay with this antibody was performed using flow cytometry on rift valley virus and Vero cells. The antibodies were added pre-infection. This antibody was used for an in vivo assay. Mice were infected with the rift valley virus. Then post infection they were given this antibody. This lead to all of the mice surviving, where the control group all died. Finally the structure of this antibody was determined using x-ray crystallography (Wang et al., 2019; PMID:30936489).

Product Class:	Purified
Clone ID:	R12
Synonyms:	GP; M polyprotein
Amount:	100 μg
Application Codes Clone:	ELISA; WB; FC; neutralizaiton; SPR; crystallography; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for the DI domain, including residues K164, H166, 173-TQEDA-177, K180, P273, K274, 291-SLK-293, and K395 of rift valley fever glycoprotein N
Application Notes:	The original version of this antibody was used for an ELISA on rift valley fever Gn and Gc. Further this antibody was used for flow cytometry on HEK293T cells transiently transfected with plasmids of pEGFP-M, pEGFP-Gn, pEGFP-Gc or pEGFP-Gn mutants. This antibody was used for a blocking assay, the rift valley virus was preincubated with this antibody and the mixture was then added to Vero cells. This antibody was used for a western blot on its linear epitope. This antibody was used for surface plasmon resonance on rift valley fever Gn and Gc. A neutralization assay with this antibody was performed using flow cytometry on rift valley virus and Vero cells. The antibodies were added pre-infection. This antibody was used for an in vivo assay. Mice were infected with the rift valley virus. Then post infection they were given this antibody. This lead to all of the mice surviving, where the control group all died. Finally the structure of this antibody was determined using x-ray crystallography (Wang et al., 2019; PMID:30936489).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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