Vector Labs Ab04464-23.0 Anti-Genome polyprotein [E106], Rabbit IgG, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: E106
Synonyms: Envelope protein E; dengue virus type I; dengue virus type 1
Amount: 100 μg
Application Codes Clone: FC; neutralization; in vitro; in vivo; SPR; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the EDIII domain of envelope protein E from dengue fever type 1.
Application Notes: The original antibody was used for flow cytometry on DENV infected Raji-DC-SIGN-R cells. It showed strong binding to DENV-1 and DENV-4, but no binding to DENV-2 and DENV-3 (Shrestha et al., 2010; PMID:20369024).This antibody was used for a plaque reduction assay on BHK21 or Vero cells and DENV-1 (Edeling et al., 2014; PMID:24743696, Shrestha et al., 2010; PMID:20369024, Shrestha et al., 2012; PMID:22406169). The structure of this antibody was determined using x-ray crystallography. An in vivo protection assay was performed on AG129 mice by administering this antibody before infection with DENV-1. It showed a significantly higher rate of survival than control. Further the in vivo study was also performed with administering this antibody after infection. Here it also showed an increase in survival (Shrestha et al., 2010; PMID:20369024). This antibody was used for surface plasmon resonance on DIII. This antibody was used for an ELISA on DENV-1. The antibody was used for a neutralization assay on Raji-DCSIGNR cells infected with DENV-1, the neutralization was tested both before and after infection had taken place (Edeling et al., 2014; PMID:24743696).

Product Class:	Purified
Clone ID:	E106
Synonyms:	Envelope protein E; dengue virus type I; dengue virus type 1
Amount:	100 μg
Application Codes Clone:	FC; neutralization; in vitro; in vivo; SPR; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the EDIII domain of envelope protein E from dengue fever type 1.
Application Notes:	The original antibody was used for flow cytometry on DENV infected Raji-DC-SIGN-R cells. It showed strong binding to DENV-1 and DENV-4, but no binding to DENV-2 and DENV-3 (Shrestha et al., 2010; PMID:20369024).This antibody was used for a plaque reduction assay on BHK21 or Vero cells and DENV-1 (Edeling et al., 2014; PMID:24743696, Shrestha et al., 2010; PMID:20369024, Shrestha et al., 2012; PMID:22406169). The structure of this antibody was determined using x-ray crystallography. An in vivo protection assay was performed on AG129 mice by administering this antibody before infection with DENV-1. It showed a significantly higher rate of survival than control. Further the in vivo study was also performed with administering this antibody after infection. Here it also showed an increase in survival (Shrestha et al., 2010; PMID:20369024). This antibody was used for surface plasmon resonance on DIII. This antibody was used for an ELISA on DENV-1. The antibody was used for a neutralization assay on Raji-DCSIGNR cells infected with DENV-1, the neutralization was tested both before and after infection had taken place (Edeling et al., 2014; PMID:24743696).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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