Mfg Part Number
Ab04466-1.29
Vector Labs Ab04466-1.29 Anti-Major capsid protein L1 [H263.A2], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with HPV-16 or HPV-18 L1 VLPs.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | H263.A2 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | L1; Human papillomavirus type 16 |
| Amount: | 100 μg |
| Application Codes Clone: | neutralization; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for an epitope with amino acids 266–297 and 339–365 being essential residues. |
| Application Notes: | The fab version of this antibody was used a neutralization assay, in short both a pre- and post-virus attachment assay was performed on 293TT cells with pseudo virus. The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:25996608). This antibody was used for an ELISA on HPV VLPs (Zhao et al., 2012; PMID:22356831, Rizk et al., 2008; PMID:18089735), and on L1-only VLPs or L1/L2 pseudo virions (Culp et al., 2007; PMID:17222883). This antibody was used for a neutralization assay on 293TT cells that were then infected with the antibody VLP mixture (Rizk et al., 2008; PMID:18089735), a similar experiment was performed on A431 cells (Culp et al., 2007; PMID:17222883). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |