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Mfg Part Number

Ab04475-2.3

Vector Labs Ab04475-2.3 Anti-Entry-fusion complex associated protein OPG095 [M12B9], Mouse IgG2a, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing a BALB/c mouse with live VACV and refolded L1 protein.

$570.70

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Description

Product Class: Purified
Clone ID: M12B9
Heavy Chain Modification: Fc Silent™
Synonyms: L1; L1R; VACV; Protein L1; OPG099; VACWR088; Virion membrane protein M25; EFC-associated protein OPG095
Amount: 100 μg
Application Codes Clone: ELISA; FC; in vivo; neutralization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for the L1 protein of the vaccina virus. Regions 25-34 and 113-131 of L1 are part of the epitope.
Application Notes: A chimeric scFv-Fc version of this antibody was used for an ELISA on recombinant protein L1 of the variola virus strain India-1967, on lysate of MV and EV forms of the vaccinia virus strain LIVP (RU2790134C1), and on overlapping linear biotinylated peptides (Kaever et al., 2014; PMID:25031354). The ability of this antibody to protect mice in vivo from VACV challenge was tested on BALB/c mice. The mice were injected with the antibody a day before they were infected with VACV-WR. The antibody is able to prevent death in mice, but it is not able to prevent weight loss (Matho et al., 2015; PMID:26325270). This antibody was used for a FACS based neutralization assay. Here Vero E6 cells were incubated with a mixture of antibody and VACV-GFP and then tested in flow cytometry (Kaever et al., 2014; PMID:25031354). This antibody was used for in vivo treatment of mice infected with VACV. It was shown that injection of this antibody after being infected with the virus lead to a 100% survival rate (Zaitseva et al., 2017; PMID:28495463).

Product Class:	Purified
Clone ID:	M12B9
Heavy Chain Modification:	Fc Silent™
Synonyms:	L1; L1R; VACV; Protein L1; OPG099; VACWR088; Virion membrane protein M25; EFC-associated protein OPG095
Amount:	100 μg
Application Codes Clone:	ELISA; FC; in vivo; neutralization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for the L1 protein of the vaccina virus. Regions 25-34 and 113-131 of L1 are part of the epitope.
Application Notes:	A chimeric scFv-Fc version of this antibody was used for an ELISA on recombinant protein L1 of the variola virus strain India-1967, on lysate of MV and EV forms of the vaccinia virus strain LIVP (RU2790134C1), and on overlapping linear biotinylated peptides (Kaever et al., 2014; PMID:25031354). The ability of this antibody to protect mice in vivo from VACV challenge was tested on BALB/c mice. The mice were injected with the antibody a day before they were infected with VACV-WR. The antibody is able to prevent death in mice, but it is not able to prevent weight loss (Matho et al., 2015; PMID:26325270). This antibody was used for a FACS based neutralization assay. Here Vero E6 cells were incubated with a mixture of antibody and VACV-GFP and then tested in flow cytometry (Kaever et al., 2014; PMID:25031354). This antibody was used for in vivo treatment of mice infected with VACV. It was shown that injection of this antibody after being infected with the virus lead to a 100% survival rate (Zaitseva et al., 2017; PMID:28495463).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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