Mfg Part Number
Ab04475-23.0
Vector Labs Ab04475-23.0 Anti-Entry-fusion complex associated protein OPG095 [M12B9], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with live VACV and refolded L1 protein.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | M12B9 |
| Synonyms: | L1; L1R; VACV; Protein L1; OPG099; VACWR088; Virion membrane protein M25; EFC-associated protein OPG095 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; FC; in vivo; neutralization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the L1 protein of the vaccina virus. Regions 25-34 and 113-131 of L1 are part of the epitope. |
| Application Notes: | A chimeric scFv-Fc version of this antibody was used for an ELISA on recombinant protein L1 of the variola virus strain India-1967, on lysate of MV and EV forms of the vaccinia virus strain LIVP (RU2790134C1), and on overlapping linear biotinylated peptides (Kaever et al., 2014; PMID:25031354). The ability of this antibody to protect mice in vivo from VACV challenge was tested on BALB/c mice. The mice were injected with the antibody a day before they were infected with VACV-WR. The antibody is able to prevent death in mice, but it is not able to prevent weight loss (Matho et al., 2015; PMID:26325270). This antibody was used for a FACS based neutralization assay. Here Vero E6 cells were incubated with a mixture of antibody and VACV-GFP and then tested in flow cytometry (Kaever et al., 2014; PMID:25031354). This antibody was used for in vivo treatment of mice infected with VACV. It was shown that injection of this antibody after being infected with the virus lead to a 100% survival rate (Zaitseva et al., 2017; PMID:28495463). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |