Mfg Part Number
Ab04479-30.11

Vector Labs Ab04479-30.11 Anti-Capsid protein VP25 [PL-2 scFv], scFv fragment (His), ScFv, (100 μg)

Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with Human astrovirus-2.
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Product Class:Purified
Clone ID:PL-2 scFv
Heavy Chain Modification:ScFv
Synonyms:Capsid polyprotein VP90; Capsid polyprotein VP70; Capsid protein VP34; Capsid protein VP27; Human astrovirus-2
Amount:100 μg
Application Codes Clone:ELISA; crystallography; WB; blocking; IF; IP
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:scFv
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody is specific for Capsid protein VP25 from Human astrovirus-2.
Application Notes:The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a western blot on HAstV-2 capsid protein (Bogdanoff et al., 2016; PMID:27213181). The original antibody was used for an ELISA on purified Spike or GFP-Spike from HAstV-2 (Bogdanoff et al., 2017; PMID:27807234, Bogdanoff et al., 2018; PMID:29070688), and on purified Human astrovirus-2 (Sanchez-Fauquier et al., 1994; PMID:7514320). Further this antibody was used for surface plasmon resonance on Spike2 from HAstV-2. This antibody has shown that it is able to block the GFP-Spike2 binding to Caco-2 cells (Bogdanoff et al., 2017; PMID:27807234). This antibody was used for immunofluorescence on Human astrovirus-2 infected LLCMK2. This antibody was used for radioimmunoprecipitation on virus samples (Sanchez-Fauquier et al., 1994; PMID:7514320). This antibody was used for a neutralization assay by preincubating human astrovirus-2 with this antibody and then adding C2Bbe1 cells (Bogdanoff et al., 2018; PMID:29070688).
More Information
Brand Vector Labs
UNSPSC 12161500