Mfg Part Number
Ab04489-23.159
Vector Labs Ab04489-23.159 Anti-Genome polyprotein [PVSP6A], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was raised by immunizing a dromedary with poliovirus type 1 Sabin strain.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | PVSP6A |
| Synonyms: | Capsid protein VP1; Capsid protein VP2; Poliovirus type 1 |
| Amount: | 100 μg |
| Application Codes Clone: | SPR; neutralization; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Dromedary VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Capsid proteins VP1 (GH loop, EF loop and C beta strand) and VP2 (EF loop) of the polio genome polyprotein. |
| Application Notes: | The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance (Thys et al., 2011; PMID:21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID:20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID:24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID:26764003). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |