Mfg Part Number
Ab04491-10.0
Vector Labs Ab04491-10.0 Anti-G [HENV-26], Human IgG1, lambda (100 μg)
Quick Overview
The original antibody was isolated from an immune human individual.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | HENV-26 |
| Synonyms: | RBP; Glycoprotein; Glycoprotein G; Attachment glycoprotein; Receptor binding protein; |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Flow cytometry; Neutralization; SPR; X-ray crystallography; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for RBP of HeV and NiV. The antibody epitope overlaps with the receptor binding sites of RBP. |
| Application Notes: | The specificity of the original format of the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The antibody could bind to the full-length RBPs by flow cytometry. The EC50 values for binding of the antibody ranged from 325 to 343 ng/mL for binding to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of the antibody in complex with both HeV-RBP and NiV-RBP were determined. The antibody protected ferrets in lethal models of infection with NiV (Dong et al., 2020; PMID: 33306954). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |