Vector Labs Ab04492-10.3 Anti-Genome polyprotein [2H4], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2H4
Heavy Chain Modification: Fc Silent™
Synonyms: JEV; E protein; Envelope protein E
Amount: 100 μg
Application Codes Clone: ELISA; Neutralization; SPR; X-ray crystallography
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for JEV. It binds a quaternary epitope that span across three adjacent envelope proteins. The antibody does not cross react with E protein from dengue (DENV1 and DENV2), Zika (ZIKV) and yellow fever (YFV) viruses.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was used in indirect immunofluoresenceThe antibody could neutralize the virus in mice (Zhang et al., 1989; PMID: 2557383). Surface plasmon resonance experiments showed that the antibody binds to the JEV rE with a high affinity of 2.9nM. The antibody could block attachment of the virus to its receptor and also prevent fusion of the virus. Cell-based neutralizing assays (in Vero cells) revealed that the antibody has strong neutralizing activities: a 50% neutralizing concentration value of 1.2 ng ml-1 against JEV (P3 strain). The antibody protected 100% of mice from lethal infection of JEV and led to complete clearance of JEV from the brains of mice, indicating its potential as a therapeutic agent. The structure of the Fab fragment of the antibody was determined by X-ray and of the Fab in complex with JEV by Cryo-EM (Qiu et al., 2018; PMID: 29379207).

Product Class:	Purified
Clone ID:	2H4
Heavy Chain Modification:	Fc Silent™
Synonyms:	JEV; E protein; Envelope protein E
Amount:	100 μg
Application Codes Clone:	ELISA; Neutralization; SPR; X-ray crystallography
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for JEV. It binds a quaternary epitope that span across three adjacent envelope proteins. The antibody does not cross react with E protein from dengue (DENV1 and DENV2), Zika (ZIKV) and yellow fever (YFV) viruses.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was used in indirect immunofluoresenceThe antibody could neutralize the virus in mice (Zhang et al., 1989; PMID: 2557383). Surface plasmon resonance experiments showed that the antibody binds to the JEV rE with a high affinity of 2.9nM. The antibody could block attachment of the virus to its receptor and also prevent fusion of the virus. Cell-based neutralizing assays (in Vero cells) revealed that the antibody has strong neutralizing activities: a 50% neutralizing concentration value of 1.2 ng ml-1 against JEV (P3 strain). The antibody protected 100% of mice from lethal infection of JEV and led to complete clearance of JEV from the brains of mice, indicating its potential as a therapeutic agent. The structure of the Fab fragment of the antibody was determined by X-ray and of the Fab in complex with JEV by Cryo-EM (Qiu et al., 2018; PMID: 29379207).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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