Vector Labs Ab04493-10.29 Anti-IL-4 [hu3B9], Human Fab fragment, His-Tagged, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: hu3B9
Heavy Chain Modification: His-Tagged
Synonyms: IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; 3B9
Amount: 100 μg
Application Codes Clone: ELISA; inhibition; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human IL-4 and binds a non-contiguous epitope.
Application Notes: The binding affinity of the original format and Fab fragment of this antibody were evaluated by ELISA. The original format of this antibody (mouse IgG1) exhibited a <0.2 nM K_d (~0.18 nM). The K_d of its Fab fragment was <0.3 nM. This antibody successfully inhibited IL-4 binding to its receptor, as evaluated using ¹²⁵ I-rhIL4 binding to the gibbon cell line, MLA. This antibody successfully inhibited lymphocyte proliferation — the original format of this antibody inhibited ³ H-thymidine incorporation by human peripheral blood T lymphocytes stimulated with 133 pM IL-4 and human tonsillar B lymphocytes stimulated by 167 pM IL-4. The IC₅₀ for inhibition of T cell proliferation was 30 pM, and for B cell proliferation 103 pM. The corresponding values for the Fab fragment were 108 and 393 pM. This antibody successfully inhibited CD23 induction — the original format of this antibody inhibited CD23 expression on human tonsil B lymphocytes stimulated with 8.3 pM IL-4 with an IC₅₀ value of 136 pM. This antibody successfully inhibited IgE secretion — the original format and the Fab fragment of this antibody inhibited IgE secretion in the presence of 1.7 nM IL-4, giving IC₅₀ values of 1.9 and 5.0 nM, respectively. The experiment was repeated using a lower concentration of IL-4, 667 pM, which reduced the IC₅₀ value to 0.65 nM for the original format of the antibody. The humanized version of this antibody effectively inhibited the binding of IL-4 receptor-Fc fusion protein to IL-4 in a dose-dependent manner — at lower concentrations (10-20 nM), it achieved 50% inhibition, while at higher concentrations (1 µM), it achieves greater than 90% inhibition. In a pharmacokinetics experiment performed on male Sprague Dawley rats, the humanized version of this antibody exhibited low inter-animal variability with a slow plasma clearance value of 0.5 mL/h/kg and an approximate 11 days half-life with a biphasic disappearance pattern (US5914110A).

Product Class:	Purified
Clone ID:	hu3B9
Heavy Chain Modification:	His-Tagged
Synonyms:	IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; 3B9
Amount:	100 μg
Application Codes Clone:	ELISA; inhibition; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human IL-4 and binds a non-contiguous epitope.
Application Notes:	The binding affinity of the original format and Fab fragment of this antibody were evaluated by ELISA. The original format of this antibody (mouse IgG1) exhibited a <0.2 nM K_d (~0.18 nM). The K_d of its Fab fragment was <0.3 nM. This antibody successfully inhibited IL-4 binding to its receptor, as evaluated using ¹²⁵ I-rhIL4 binding to the gibbon cell line, MLA. This antibody successfully inhibited lymphocyte proliferation — the original format of this antibody inhibited ³ H-thymidine incorporation by human peripheral blood T lymphocytes stimulated with 133 pM IL-4 and human tonsillar B lymphocytes stimulated by 167 pM IL-4. The IC₅₀ for inhibition of T cell proliferation was 30 pM, and for B cell proliferation 103 pM. The corresponding values for the Fab fragment were 108 and 393 pM. This antibody successfully inhibited CD23 induction — the original format of this antibody inhibited CD23 expression on human tonsil B lymphocytes stimulated with 8.3 pM IL-4 with an IC₅₀ value of 136 pM. This antibody successfully inhibited IgE secretion — the original format and the Fab fragment of this antibody inhibited IgE secretion in the presence of 1.7 nM IL-4, giving IC₅₀ values of 1.9 and 5.0 nM, respectively. The experiment was repeated using a lower concentration of IL-4, 667 pM, which reduced the IC₅₀ value to 0.65 nM for the original format of the antibody. The humanized version of this antibody effectively inhibited the binding of IL-4 receptor-Fc fusion protein to IL-4 in a dose-dependent manner — at lower concentrations (10-20 nM), it achieved 50% inhibition, while at higher concentrations (1 µM), it achieves greater than 90% inhibition. In a pharmacokinetics experiment performed on male Sprague Dawley rats, the humanized version of this antibody exhibited low inter-animal variability with a slow plasma clearance value of 0.5 mL/h/kg and an approximate 11 days half-life with a biphasic disappearance pattern (US5914110A).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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