Vector Labs Ab04494-10.0 Anti-IL-13 [6A1], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 6A1
Synonyms: IL13; Interleukin-13; P600
Amount: 100 μg
Application Codes Clone: ELISA; inhibition; neutralization; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13.
Application Notes: The original format of this antibody (mouse IgG1) showed binding activity against E.coli-expressed recombinant human IL-13 in a sandwich ELISA with an EC₅₀ of 0.049 and 0.039 µg/ml for human and cynomolgus IL-13. The antibody also exhibited potent binding and neutralization activities against various forms of IL-13. In inhibition assays, the antibody showed IC₅₀ values of 0.165 µg/ml for blocking human IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2. Moreover, it demonstrated strong neutralization capabilities with ND₅₀ (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1 cell proliferation (where the ND₅₀ value is the concentration of monoclonal antibody required to reduce TF-1 cell proliferation by 50%, in response to a set concentration of IL-13). Furthermore, the antibody effectively neutralized mammalian-expressed human IL-13 with an ND 50 value of 0.037 µg/ml and a recombinant Q130 human IL-13 variant with an ND₅₀ value of 0.110 µg/ml. The binding affinity of the antibody was measured by SPR and found to be 32, 270, and 61.2 pM for det-1 tagged human IL-13, human IL-13, and cynomolgus IL-13, respectively (EP1802658B1).

Product Class:	Purified
Clone ID:	6A1
Synonyms:	IL13; Interleukin-13; P600
Amount:	100 μg
Application Codes Clone:	ELISA; inhibition; neutralization; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13.
Application Notes:	The original format of this antibody (mouse IgG1) showed binding activity against E.coli-expressed recombinant human IL-13 in a sandwich ELISA with an EC₅₀ of 0.049 and 0.039 µg/ml for human and cynomolgus IL-13. The antibody also exhibited potent binding and neutralization activities against various forms of IL-13. In inhibition assays, the antibody showed IC₅₀ values of 0.165 µg/ml for blocking human IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2. Moreover, it demonstrated strong neutralization capabilities with ND₅₀ (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1 cell proliferation (where the ND₅₀ value is the concentration of monoclonal antibody required to reduce TF-1 cell proliferation by 50%, in response to a set concentration of IL-13). Furthermore, the antibody effectively neutralized mammalian-expressed human IL-13 with an ND 50 value of 0.037 µg/ml and a recombinant Q130 human IL-13 variant with an ND₅₀ value of 0.110 µg/ml. The binding affinity of the antibody was measured by SPR and found to be 32, 270, and 61.2 pM for det-1 tagged human IL-13, human IL-13, and cynomolgus IL-13, respectively (EP1802658B1).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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