Mfg Part Number
Ab04495-12.1
Vector Labs Ab04495-12.1 Anti-gB [1G2], Human IgG3, lambda (100 μg)
Quick Overview
The original antibody was isolated from EBV transformed human peripheral blood derived B cells derived from hCMV-infected donors.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1G2 |
| Synonyms: | Envelope glycoprotein B; UL55; Ab-50 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Crystallography; in vitro; neutralization; SPR; FC; FACS; IP |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 26365435 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for a surface epitope in antigenic domain 5 (AD-5), also termed Domain I (Dom I), of Human cytomegalovirus (hCMV) (strain Towne), also called Human herpesvirus 5 (HHV-5). Dom I resides between amino acid residues 133 to 343 of the gB of hCMV strain AD169 and has the following sequence: IVAHTFKVRVYQKVLTFRRSYAYIYTTYLLGSNTEYVAPPMWEIHHINKFAQCYSSYSRVIG GTVFVAYHRDSYENKTMQLIPDDYSNTHSTRYVTVKDQWHSRGSTWLYRETCNLNCMLT ITTARSKYPYHFFATSTGDVVYISPFYNGTNRNASYFGENADKFFIFPNYTIVSDFGRPNAA PETHRLVAFLERADSVISWDIQDEKNVT. The exact epitope lies between Tyr280-Phe300, or: Y-NGTNRNASYFGENADKFFIF-P. |
| Application Notes: | The original version of the antibody was used in a neutralization assay against human cytomegalovirus (hCMV). It was found to neutralize hCMV with high potency — it neutralized the virus with an IC50 of 0.1 μg/ml and an IC90 of 0.4 μg/ml (US9346874B2). The Fab version of this antibody was generated and its structure in complex with hCMV gB was explored using crystallography. It was also used in neutralization assays, surface staining of full-length gB in flow cytometry, and gB immunoprecipitation (Chandramouli et al., 2015; PMID: 26365435). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |