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Mfg Part Number

Ab04497-23.0

Vector Labs Ab04497-23.0 Anti-Glycoprotein C [1C11], Rabbit IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with SBV strain BH80/11.

$570.70

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Description

Product Class: Purified
Clone ID: 1C11
Synonyms: Gc; SBV; M polyprotein; Glycoprotein G1; Envelopment polyprotein
Amount: 100 μg
Application Codes Clone: SPR; Neutralizing; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for the envelopment polyprotein of SBV. It binds to a conformational epitope on the apical end of the head domain near the N686 glycosylation site. Its epitope has an area of 907 A2 and is centered on the third disulfide bond, with contributions from the regions around helices h8–h10 and h12–h13.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody reacted with SBV by indirect immunofluorescence (Wernike et al., 2015; PMID: 25889366). The binding affinity of the original format of the antibody to the recombinant SBV head–stalk construct was measured by surface plasmon resonances (KD = 5.4 ± 0.3 nM). The original format of the antibody neutralized SBV on Vero cells in a focus reduction neutralization (FRNT) assay with ND50 = 0.15 ± 0.01 µM. The scFv format of the antibody was co-crystallized in complex with the SBV Gc head domain. The original format of the antibody could protect IFNAR−/− mice from lethal SBV infection in vivo (Hellert et al., 2019; PMID: 30787296).

Product Class:	Purified
Clone ID:	1C11
Synonyms:	Gc; SBV; M polyprotein; Glycoprotein G1; Envelopment polyprotein
Amount:	100 μg
Application Codes Clone:	SPR; Neutralizing; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for the envelopment polyprotein of SBV. It binds to a conformational epitope on the apical end of the head domain near the N686 glycosylation site. Its epitope has an area of 907 A2 and is centered on the third disulfide bond, with contributions from the regions around helices h8–h10 and h12–h13.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody reacted with SBV by indirect immunofluorescence (Wernike et al., 2015; PMID: 25889366). The binding affinity of the original format of the antibody to the recombinant SBV head–stalk construct was measured by surface plasmon resonances (KD = 5.4 ± 0.3 nM). The original format of the antibody neutralized SBV on Vero cells in a focus reduction neutralization (FRNT) assay with ND50 = 0.15 ± 0.01 µM. The scFv format of the antibody was co-crystallized in complex with the SBV Gc head domain. The original format of the antibody could protect IFNAR−/− mice from lethal SBV infection in vivo (Hellert et al., 2019; PMID: 30787296).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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