Vector Labs Ab04500-23.0 Anti-CD11a [AL-57], Rabbit IgG, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AL-57
Synonyms: LFA-1A; integrin LFA-1; Integrin alpha-L; activated LFA-1 clone 57; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain ;Leukocyte function-associated molecule 1 alpha chain
Amount: 100 μg
Application Codes Clone: ELISA; FC; SPR; Crystallization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody binds to the alpha L I domain in a HA but not LA conformation.The antibody binding site overlaps the ICAM-1 binding site on the I domain.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The cell-binding specificity of the original and IgG1 format of the antibody was determined using flow cytometric analysis with HA and LA cells, the antibody bound to HA cells in a dose-dependent manner but did not bind to LA cells. The antibody preferentially binds to the active conformation of LFA-1 and blocks LFA-1-mediated adhesion and lymphocyte proliferation (Huang et al., 2006; PMID: 16888085). The original format of the antibody could discriminate among low-affinity, intermediate-affinity (IA), and HA states of LFA-1. Furthermore, the binding of the antibody depended on the presence of Mg2+. The antibody showed no binding to the WT I domain, intermediate binding to the IA I domain (KD = 4.7 μM), and good binding to the HA I domain by SPR (KD = 0.023 μM). The original format of the antibody showed affinity increases on a subset (≈10%) of lymphocyte cell surface LFA-1 molecules upon stimulation with CXC chemokine ligand 12 (CXCL-12). The IgG1 format of the antibody could bind to HA I domain expressed on the surface of K562 cells presence of Mg2+, as tested by using immunofluorescent flow cytometry (Shimaoka et al., 2006; PMID: 16963559). The crystal structures of the Fab fragment of the antibody alone and in complex with IA were determined (Zhang et al., 2009; PMID: 19805116).

Product Class:	Purified
Clone ID:	AL-57
Synonyms:	LFA-1A; integrin LFA-1; Integrin alpha-L; activated LFA-1 clone 57; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain ;Leukocyte function-associated molecule 1 alpha chain
Amount:	100 μg
Application Codes Clone:	ELISA; FC; SPR; Crystallization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody binds to the alpha L I domain in a HA but not LA conformation.The antibody binding site overlaps the ICAM-1 binding site on the I domain.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The cell-binding specificity of the original and IgG1 format of the antibody was determined using flow cytometric analysis with HA and LA cells, the antibody bound to HA cells in a dose-dependent manner but did not bind to LA cells. The antibody preferentially binds to the active conformation of LFA-1 and blocks LFA-1-mediated adhesion and lymphocyte proliferation (Huang et al., 2006; PMID: 16888085). The original format of the antibody could discriminate among low-affinity, intermediate-affinity (IA), and HA states of LFA-1. Furthermore, the binding of the antibody depended on the presence of Mg2+. The antibody showed no binding to the WT I domain, intermediate binding to the IA I domain (KD = 4.7 μM), and good binding to the HA I domain by SPR (KD = 0.023 μM). The original format of the antibody showed affinity increases on a subset (≈10%) of lymphocyte cell surface LFA-1 molecules upon stimulation with CXC chemokine ligand 12 (CXCL-12). The IgG1 format of the antibody could bind to HA I domain expressed on the surface of K562 cells presence of Mg2+, as tested by using immunofluorescent flow cytometry (Shimaoka et al., 2006; PMID: 16963559). The crystal structures of the Fab fragment of the antibody alone and in complex with IA were determined (Zhang et al., 2009; PMID: 19805116).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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