Mfg Part Number
Ab04515-23.159
Vector Labs Ab04515-23.159 Anti-TLR4 [107-H7], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing llamas with a cocktail of murine and human Toll-like receptors (TLRs) that contained recombinant human TLR4/MD2, recombinant human TLR3, recombinant murine TLR3, and recombinant murine TLR2/Fc chimera. Peripheral blood mononuclear cells were isolated from the animals' blood for the construction of a phage display library, and the library was panned against the TLRs used for immunization.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 107-H7 |
| Synonyms: | hToll; CD284; Toll-like receptor 4 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Agnostic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Llama VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human TLR4. |
| Application Notes: | The binding specificity of the original version of this antibody (VHH) was tested using ELISA. In a cell-based neutralizing assay with hTLR4-overexpressing HEK cells, an agonistic effect was observed, as measured by increased NFκB expression after cell lysis compared to the control group, indicating activation or enhancement of TLR activity instead of neutralization (WO2009138494A2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |