Vector Labs Ab04517-23.0 Anti-M-CSF [Caline], Rabbit IgG, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Caline
Synonyms: MCSF; CSF1; Macrophage colony-stimulating factor; Macrophage colony-stimulating factor 1; Lanimostim; Proteoglycan macrophage colony-stimulating factor 1; PG-M-CSF 1
Amount: 100 μg
Application Codes Clone: ELISA; FACS; Inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types.
Application Notes: The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. K_D values of 14 pM and 16 pM were measured. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC₅₀ value of 9.8 nM and 14 nM against M-CSF-expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC₅₀ of 6.1 pM and 6.3 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC₅₀ of 3.1 pM and 17.7 pM for recombinant and native human M-CSF, respectively, and 4.3 pM and 106.9 pM for recombinant and native cynomolgus M-CSF, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by membrane-bound human M-CSF (expressed by stably transfected CHO cells) (US10081675B2).

Product Class:	Purified
Clone ID:	Caline
Synonyms:	MCSF; CSF1; Macrophage colony-stimulating factor; Macrophage colony-stimulating factor 1; Lanimostim; Proteoglycan macrophage colony-stimulating factor 1; PG-M-CSF 1
Amount:	100 μg
Application Codes Clone:	ELISA; FACS; Inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types.
Application Notes:	The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. K_D values of 14 pM and 16 pM were measured. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC₅₀ value of 9.8 nM and 14 nM against M-CSF-expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC₅₀ of 6.1 pM and 6.3 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC₅₀ of 3.1 pM and 17.7 pM for recombinant and native human M-CSF, respectively, and 4.3 pM and 106.9 pM for recombinant and native cynomolgus M-CSF, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by membrane-bound human M-CSF (expressed by stably transfected CHO cells) (US10081675B2).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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