Mfg Part Number
Ab04532-1.1
Vector Labs Ab04532-1.1 Anti-Gintexin A type PLA2 [N20 (3C12)], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was prepared by immunizing BALB/c mice with attenuated Gintexin antigen purified from the crude venom of Gloydius intermedius.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | N20 (3C12) |
| Synonyms: | Gintexin A; PLA2; phospholipase 2; GA-3C12 |
| Amount: | 100 μg |
| Application Codes Clone: | WB; ELISA; neutralize |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds the gintexin A type PLA2 protein found in the venom of Gloydius intermedius. Gintexin A is Asn-49 type PLA2 isomer that has no enzymatic activity and neurotoxicity. Gentexin is a heterodimer comprising gintexin A and gentexin B. It is estimated that, after the venom enter the blood system GintexinA acts as a "director" to guide the neurotoxin heterodimer to bind to the membrane receptor at the phrenic nerve NMJ synapse, and then Gintexin B exerts neurotoxicity (blocks the diaphragm NMJ synaptic transmission and stops breathing). |
| Application Notes: | This antibody was capable of recognizing attenuated gintexin A of Gloydius intermedius and a fusion protein containing gintexin A in a western blot assay. The binding affinity of this antibody for gintexin A was determined using ELISA. 100 μg this antibody can neutralize at least 2×LD50 of Agkistrodon venom. In vivo, the neutralizing activity of this antibody is reported to be about 21.1 LD50/mg. The sensitivity of HRP-N20 antibody for immunospot detection of Agkistrodon intermedius venom is 12.5 ng/mL. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |