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Mfg Part Number

Ab04542-23.0

Vector Labs Ab04542-23.0 Anti-integrin alpha-5 beta-1 [339.1], Rabbit IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing a Female Sprague-Dawley rat with alpha-5 beta-1 purified from mouse placenta or with a mouse alpha-5 beta-1-Fc fusion protein

$570.70

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Description

Product Class: Purified
Clone ID: 339.1
Synonyms: Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29; Itga5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; CD49e; VLA-5
Amount: 100 μg
Application Codes Clone: ELISA; IHC; in vivo; in vitro; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for alpha-5 beta-1
Application Notes: The original antibody was used for an ELISA on alpha-5 beta-1-Fc fusion protein. The original antibody was used for surface plasmon resonance on alpha-5 beta-1-Fc. It showed a binding affinity of 0.59 nM. This antibody was used for an Annexin V cell death assay. Furthermore this antibody was used for immunohistochemistry on Xenograft tumours derived from cells inoculated subcutaneously in ICR-SCID or SCID-Beige mice (Bhaskar et al., 2007; PMID:18042290). This antibody was used to test the effect of alpha-5 and beta-1 integrin blockers on OvCa cell lines HeyA8 and SKOV3ip1. Further this antibody was used for in vivo testing of alpha-5 and beta-1 integrin blocking antibodies using the HeyA8 xenograft model of OvCa metastasis. It showed a decrease in the number of tumours in the subjects (Kenny et al., 2014; PMID:25202979).

Product Class:	Purified
Clone ID:	339.1
Synonyms:	Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29; Itga5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; CD49e; VLA-5
Amount:	100 μg
Application Codes Clone:	ELISA; IHC; in vivo; in vitro; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Rat Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for alpha-5 beta-1
Application Notes:	The original antibody was used for an ELISA on alpha-5 beta-1-Fc fusion protein. The original antibody was used for surface plasmon resonance on alpha-5 beta-1-Fc. It showed a binding affinity of 0.59 nM. This antibody was used for an Annexin V cell death assay. Furthermore this antibody was used for immunohistochemistry on Xenograft tumours derived from cells inoculated subcutaneously in ICR-SCID or SCID-Beige mice (Bhaskar et al., 2007; PMID:18042290). This antibody was used to test the effect of alpha-5 and beta-1 integrin blockers on OvCa cell lines HeyA8 and SKOV3ip1. Further this antibody was used for in vivo testing of alpha-5 and beta-1 integrin blocking antibodies using the HeyA8 xenograft model of OvCa metastasis. It showed a decrease in the number of tumours in the subjects (Kenny et al., 2014; PMID:25202979).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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