Mfg Part Number
Ab04543-23.0
Vector Labs Ab04543-23.0 Anti-Integrin alpha-L [D2-57], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was raised using human phage display library FAB-300. To screen the library purified locked-open HA, locked-closed LA, and wild-type I domains of LFA-1 were used.
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| Product Class: | Purified |
|---|---|
| Clone ID: | D2-57 |
| Synonyms: | AL-57; CD11a; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR; FC; in vitro; IF; crystallography |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the LFA-1 I domain in a HA conformation. |
| Application Notes: | The original antibody was used for an ELISA on LFA-1 HA I domain. This antibody was used for immunofluorescence on K562 cells with different LFA-1 conformations. Furthermore this antibody was used for flow cytometry on K562 cells with different LFA-1 conformations (Huang et al., 2006; PMID:16888085), and on IL-15-cultured T lymphocytes (Shimaoka et al., 2006; PMID:16963559). This antibody was used for an ICAM-1 blocking assay on HA cells, to determine the effect of the antibody to inhibit ligand binding. The antibody was used for a PHA-induced lymphocyte proliferation assay on PBMCs, to determine the effect of the antibody on these cells. It did indeed block PHA-induced lymphocyte proliferation (Huang et al., 2006; PMID:16888085). This antibody was used for surface plasmon resonance on LFA-1 I domain to determine its binding affinity (Shimaoka et al., 2006; PMID:16963559). The structure of this antibody was determined using x-ray crystallography (Zhang et al., 2009; PMID:19805116). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |