Mfg Part Number
Ab04557-2.3
Vector Labs Ab04557-2.3 Anti-CD40 ligand [5c8], Mouse IgG2a, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a BALB/c mouse with D1.1 cells.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 5c8 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD40LG; TRAP; CD154; CD40 ligand; CD40L; TNFSF5, tumor necrosis factor ligand superfamily member 5; tumor necrosis factor related activation protein |
| Amount: | 100 μg |
| Application Codes Clone: | catalysis; crystallography; FC; IP; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for human CD40 ligand. |
| Application Notes: | The original version of this antibody was used to catalyse disfavoured endo-tet cyclization reactions of trans-epoxy alcohols (Gruber et al., 1999; PMID:10353817). The structure of the fab version of the antibody was determined using x-ray crystallography (Gruber et al., 1999; PMID:10353817, Karpusas et al., 2001; PMID:11525169). The original antibody was used for flow cytometry on D1.1 cells. It only showed binding to D1.1 cells but not to B2.7 cells. This antibody was used for immunoprecipitation on Jurkat cell lysates and precipitated a 30kDa protein. This antibody also shows the ability to inhibit D1.1 cell induced B cell activation (Lederman et al., 1992; PMID:1348081). The half life of this antibody in cynomolgus monkeys was measured. The data suggested a half life between 300-500 hours. This antibody was tested in vivo on cynomolgus monkeys. When first given this antibody and then performing a tetanus toxoid challenge it decreased antibody titres (Gobburu et al., 1998; PMID:9694951). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |